SUBJECT

Title

Chemical and Functional Characterisation of Biologically Active Peptides in vitro.

Type of instruction

lecture + laboratory

Level

Master

Part of degree program
Credits

1+1

Recommended in

Semester 1

Typically offered in

Autumn semester

Course description

Chemical characterisation of peptides and peptide derivatives (amino acid analysis, sequencing, determination of the substitution degree). Elemental analysis, determination of fluorescence parameters. Cellular uptake studies (concentration, time- and temperature dependency). Cytotoxicity studies.

Readings

Compulsory literature*:

  • Cooper, C: Amino Acid Analysis Protocols, (chapters) Humana Press, 2000

  • Walker, JM: Basic Protein and Peptide Protocols, (chapters) Humana Press, 1994

  • Walker, JM: Protein Protocols, (chapters) Humana Press, 2002