Hyphenated Techniques for Element Speciation

Type of instruction






Recommended in

Semester 2/4

Typically offered in

Spring semester

Course description

General characterization of off-line (HPLC-GF-AAS, HPLC-TXRF, TLC/OPLC-TXRF, TLC-LA-ICP-MS) and on-line techniques (HPLC-FAAS, HPLC-ICP-AES, HPLC-ICP-MS, GC-AAS, LC-QF-AAS) for element speciation in environmental chemistry; speciation of As, Cd, Cr, Hg, Ni, Pb and Sn in environment. Detailed course outline: 1. Reasons for doing elemental speciation; 2. Sampling strategies for elemental speciation: sampling of environmental samples; 3. Sampling strategies for elemental speciation: sampling of clinical samples; 4. Sample preparation for elemental speciation: derivatization and pre-concentration methods;5. Hyphenation techniques for volatile samples (GC as separation technique); 6. Hyphenation techniques for liquid samples (HPLC and CE as separation techniques); 7. Speciation techniques for solid samples: X-ray diffraction and XANES; 8. Toxicity of inorganic and organic arsenic species;9. Arsenic speciation in plant compartments; 10. Organotin compounds: from their discovery to their use as antifouling paints; 11. Toxicity of organotin compounds; 12. Toxicity of inorganic and organic mercury compounds. Minamata disease; 13. Biomonitoring for mercury exposure; 14. Lead toxicity. Saturnism;15. Toxicokinetics of lead;16. Biomarkers of lead exposure; 17. Speciation of chromium.

  • Handbook of Elemental Speciation (Eds.: R. Kertulis, H. Crews, J. Caruso, K.G. Heumann), Wiley, 2005

  • Modern Methods for Trace Element Determination (Eds.: C. Vandecasteele and C.B. Block), Wiley, 1995

  • Practical HPLC method development (Eds.: L.R. Snyder, J.J. Kirkland, Glajch J.L.), Wiley, 1997

  • Atomic Absorption and Plasma Spectrometry (Ed.: J. R. Dean), Wiley, 1997