SUBJECT
Light Microscopic Microtechniques for Plant Materials
practical
master
4
Semesters 1-4
Autumn/Spring semester
1. Fundamentals of light microscopy
2. Special light mictoscopy techniques
3. Sample preparation, fixation, types and effects of fixatives.
4. Dehydration, comparison of different dehydrating agents, paraffin infiltration.
5. PEG technique, acryl based embedding materials.
6. Michrotomes, glass and metal knives.
7. Sectioning of different materials.
8. Staining paraffin and plastic sections.
9. Mounting and preserving sections.
10. Special techniques, whole mounts, maceration.
11. preparation of samples for fluorescent microscopy.
12. In situ techniques.
13. Immunocitology.
14. Histo-, and citochemistry.
15. Kryotechnique.
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John D. Bancroft, Marilyn Gamble: Theory and Practice of Histological Techniques, Elsevier Health Sciences, 2008