The aim of the course is to introduce the students into mass spectrometry, one of the most important methods in analytical chemistry.
1) Basics of mass spectrometry (definitions, isotope pattern, accurate mass)
2) Ionization techniques: 2.a) EI ionization (fragmentation rules, spectrum elucidation), classical applications 2.b) Spray techniques (Electrospray, APCI, APPI) 2.c) MALDI
3) Analysers (MS instrumentation)
4) Tandem mass spectrometry
5) Hyphenated techniques (GC–MS, HPLC–MS)
6) Application of mass spectrometry in diverse fields, including organic, bioorganic pharmaceutical applications, proteomics, food and environmental analysis, medical diagnostics, materials science
Fred W. McLafferty, F. Turecek, Interpretation of Mass Spectra, 4th ed., University Science Books, Sausalito, 1994.
John Greaves, John Roboz, Mass Spectrometry for the Novice, CRC Press, Boca Raton, 2013
Vékey, Telekes, Vértes (editors), Medical Applications of Mass Spectrometry, Elsevier, Amsterdam, 2007
J. R. chapman. Practical organic mass spectrometry. A guide of chemical and biochemical analysis. Wiley, Chichester, 2nd edn, 1993.