Organic Spectroscopy Laboratory
The aim of the course is to teach students the most important instrumental methods used in the structural investigation of organic compounds and biomolecules. Besides learning the basic methodology, the students are performing complex measurements which reveal the applicability and limitations of the studied analytical methods. The wide range of covered investigation techniques is aimed at developing the critical sense of students in choosing the most appropriate analytical procedure for solving a concrete problem and to be able to determine the structure of an unknown compound by combining several structural analysis methods. The course also gives an introduction to the stereochemical structure analysis of biomolecules. The subject includes ultraviolet-visible (UV-VIS), infrared (IR), NMR, circular dichroism (CD), vibrational optical activity (VCD and ROA), as well as organic and biomolecular mass spectrometry laboratory courses.
Joseph B. Lambert, Herbert F. Shurvell, David A. Lightner, R. Graham Cooks: Organic Structural Spectroscopy, Prentice Hall, Upper Saddle River, New Jersey, USA (2001).
Suggested literature: L.D. Field, S. Sternhell, J.R. Kalman: Organic Structures from Spectra, third edition, John Wiley & Sons, Chichester, UK (2002)