SUBJECT

Title

PCR Techniques in Plant Molecular Biology II

Type of instruction

practical

Level

master

Faculty

Faculty of Science

Part of degree program

Biology MSc

Credits

Recommended in

Semesters 1-4

Typically offered in

Autumn/Spring semester

Course description

1. DNA extraction and purification from wheat (variety Chinese Spring, Glenlea, Bánkúti 306) and analysis with spectrophotometer and gel electrophoresis. 4 hours

2. Nested PCR for genes expressed only in the endosperm (HMW glutenin subunit), and Simple-Sequence Repeats (SSR; Bx7 vs. Bx7 ) . 6 hours

3. Using Single Nucleotide Polymorphism (SNP; Dx2 vs. Dx5) markers for identification of different HMW glutenin subunit genes. 6 hours

4. Nested PCR second step 4 hours

5. QRT-PCR for studying the change in transcription level of the storage proteins during seed development. 10 hours

Readings

Weising, Nybom, Wolff Kahl et al.: DNA Fingerprinting in Plants, CRC Press, 2005
+ Review articles in English