SUBJECT
Title
Plant Cell and Tissue Culturing II
Type of instruction
practical
Level
master
Faculty
Part of degree program
Credits
2
Recommended in
Semesters 1-4
Typically offered in
Autumn/Spring semester
Course description
1-2) Preparing of culture medium, sterilization, pourring into vessels
3-4) Initiation and maintenance of callus and cell suspension cultures, microscopical investigations on cell types of the cultures
5-6) Inocule sterilization, initiation of embryo cultures
7-8) Visiting a cell fermentation laboratory,
9-10) Isolation of protoplasts, protoplast fusin by PEG
11-12) Micropropagation, rooting
13-14) Use of biolistic (gene) gun
Readings
-
Roberta H. Smith: Plant Tissue Culture: Techniques and Experiments, Academic Press, 2006
-
Victor M. Loyola-Vargas, Felipe Vázquez-Flota: Plant Cell Culture Protocols, Springer Science & Business Media, 2006