Bacterial and (New) Phage Genetics
1. The importance of early phage genetics: The DNA as the hereditary material, Avery experiment, Hershey-Chase experiment. The identification of restriction and modification. Deciphering the "code".
2. The T4 model system: Phage experimental methods. Early genes. Late genes. Regulation of gene expression, timing. Virulent phages.
3. The genetics of temperate phages: Lysogeny. Maintaining the lysogenic status. Lysogen-virulent transition. Integration, excision.
4. Regulatory systems: Regulation of transcription. Timing with antitermination.
5. Transduction: Introduction of DNA with transduction. Specialized and general transduction
6. Transformation: Pneumococcus, Bacillus transformation. Non-natural transformation. Competence.
7. Conjugation: F plasmid. Conjugative plasmids
8. Plasmids: Chromosome mobilizing plasmids. Bacteriocins. Resistance plasmids.
9. Applied phage genetics: Recombinant DNA technology, vectors: Cloning vectors. Replacement and insertion vectors
10. Recombination, integration systems: Site-specific recombination. Illegitim recombination. Cambell type recombination.
11. Microbial plant cell wall degradation: Structure and composition of the plant cell wall. Aerobic and anaerobic systems. Genes involved in cell wall degradation
12. New phage applications (New phage genetics): Medical applications. Phage display. Phage two-hybrid system
13. Bacteriophage evolution in the frame of genome programmes: Mosaic genome building. The lack of molecular clock. Structural/proteome evlution
14. Bacterial evolution: Genome size. The importance of lateral gene transfer. Molecular taxonomy (identification Archaea, Eubacteria). The importance of transposons and phages in genome evolution. The significance of resistance plasmids.
E. A. Birge: Bacterial and bacteriophage genetics, 4th ed., Springer Science & Business Media, 2013, ISBN 9781475732580