SUBJECT

Biotechnology

lecture

master

Faculty of Science

Biology MSc

3

Semesters 1-4

Autumn/Spring semester

1. Introduction: from classical techniques to modern biotechnology

2. DNA reactions: replication, repair, mutation, recombination.

3. Restriction-modification, DNA characterization with endonucleases, identification, physical mapping, DNA, hybridization.

4. Genetic information transfers: transformation, transduction, conjugation.

5-6. Gene cloning: selection of gene-DNA, endonuclease fragment, c-DNA preparation, DNA synthesis, selection from gene library.

7. Vectors: plasmids, phages, cosmid vectors, preparation and use of a gene library.

8. Identification and characterization of gene clones, expression, regulation, stability.

Microorganisms in agriculturalbiotechnology.

9. Biological nitrogen fixation biochemical principles, nitrogenase enzyme, nitrogen assimilation.

10. Free living, symbiotic and associative nitrogen fixation, genes and regulation involved in plant-bacterium interactions, practical application, soil inoculants.

11. Biological plant protection microbes and viruses against insects, pests, microbial weed control, hormon stimulation producers.

12. Animal feeding, Production of foder additiv antibiotics and amino acids, silage, SCP.

13. Bioconversion, bioremediation, detoxification:, antinutritives, lignin, cellulose, hemicellulose decomposition and utilization, industrial wastes, plasmid coded decomposition of toxic wastes, specialized gene manipulated microorganisms.

14. Vaccines and therapeutic agents.

• B. R. Glick and J. J. Pasternak: Molecular Biotechnology Princilples and Applications of Recombinant DNA. ASM Press Washington DC 1994.

• D.Watson: Recombinant DNA: Genes and Genomes, W. H. Freeman, 2007