SUBJECT

Classical and molecular bacterium taxonomy II

practical

master

Faculty of Science

Biology MSc

4

Semesters 1-4

Autumn/Spring semester

1. Phenetic clustering techniques based on rapid diagnostic techniques. Conventions in microbiology.

2. Investigation of cell-wall and cell membrane chemotaxonomical markers (characteristic amino acids, and sugars from whole cell hydrolysate, diversity of quinones, PLFA analysis)

3. Amplification of 16S rDNA by PCR, partial and full sequence analysis (DNA extraction from a strain, PCR; extraction of NAS from environmental samples, PCR vs. RT-PCR, gene cloning)

4. Analysis of sequence data, use of ARB and other phylogenetic software (problems of tertiary structure, fingerprint sequences)

5. Reconstruction of phylogenetic tree based on local and web data-bases. Development of databases for taxonomic and ecological purposes, in-silico taxonomy.

6. Analysis of microevolution events based on ITS, VNTR techniques.

• Towner K.J.-Cockayne A.: Molecular Methods for Microbial Identification and Typing. Chapman & Hall, London, 1995

• Stackebrandt E. (ed.): Molecular identification, systematics and population structure of prokaryotes. Springer-Verlag, Berlin, 2006