Classical and Molecular Biological Methods in Microbiology
1. Extraction of total DNA from an environmental sample. Consensus PCR on molecular chronometers. Assessment of species richness by restriction digestion (ARDRA, T-RFLP)
2. Use of molecular fingerprint techniques for taxonomy and ecology purposes. FAME analysis. Extraction of respiratory quinones, and their use in population dynamics studies.
3. Chemotaxonomy techniques: analysis of cell-wall markers. Fermentation end product analysis.
4. Biological water qualification. Microscopic analysis of indicator microorganisms. Use of chlorophyll-a determination to assess trophity level.
5. Analysis of DNA sequence data of chronometer genes. Tree construction.
6. Use of rapid diagnostic techniques in animal, and/or plant microbe interaction studies.
C. A. Reddy, Terry J. Beveridge, John A. Breznak, George Marzluf: Methods for General and Molecular Microbiology, American Society for Microbiology Press, 2007