SUBJECT

Classical and Molecular Biological Methods in Microbiology

practical

master

Faculty of Science

Biology MSc

4

Semester 3

Autumn semester

1. Extraction of total DNA from an environmental sample. Consensus PCR on molecular chronometers. Assessment of species richness by restriction digestion (ARDRA, T-RFLP)

2. Use of molecular fingerprint techniques for taxonomy and ecology purposes. FAME analysis. Extraction of respiratory quinones, and their use in population dynamics studies.

3. Chemotaxonomy techniques: analysis of cell-wall markers. Fermentation end product analysis.

4. Biological water qualification. Microscopic analysis of indicator microorganisms. Use of chlorophyll-a determination to assess trophity level.

5. Analysis of DNA sequence data of chronometer genes. Tree construction.

6. Use of rapid diagnostic techniques in animal, and/or plant microbe interaction studies.

• C. A. Reddy, Terry J. Beveridge, John A. Breznak, George Marzluf: Methods for General and Molecular Microbiology, American Society for Microbiology Press, 2007