Experimental Methods in neurobiology II.
Miscellenous imaging techniques: FMRI, MRI, CT PET
Taking samples from brain tissue: Microdissection Micro-punch Laser capture Basics of sample preparation
Methods of neurochemistry Binding-assay Cell-frakcioning, studies on liposomes, sinaptosomes Stop-flow, receptor kinetics Izotop technique Fluorescent tags Microdialysis Analythical HPLC: theories, equipment, columns, eluents Measuerement of bioactive compounds from the extracellular space (monoamines, amino acids, nucleosides, peptids) LC-MS, electrospray ionization (ESI), field of application
Systems biology techniques Gen chipping Data base search and in silico biology Gen hybridization on chip, chip-PCR Planning of experiments Separation: 2D-electrophoresis, differencial electrophoresis (DIGE) Sample praparation, digestion (manual, automatic) Protein identification: MS: equipment: MALDI, SELDI, Tandem MS, Q, Q-TOF, Q-TOF-TOF MS sequenation, protein identification Protein network modeling: CYTOSCAPE, interaction databases
G.B. Smejkal, A. Lazarev Separation Methods in Proteomics, Taylor&Francis, NY, 2006
S. Srivastava Informatics in Proteomics, Taylor&Francis, NY, 2005
J.H. Gross Mass Spectrometry, Springer, 2004