SUBJECT
In vitro Cell Technology
lecture
master
2
Semesters 1-4
Autumn/Spring semester
1. Short history of cell and tissue culturing; different types of cultures; applications of cell culture techniques
2. The fluid environment of cultivated cells
3. The “solid” environment of cells in vitro and in vivo. The basic features of cell adhesion and migration
4. Cell adhesion molecules; The cytoskeleton; Factors determining the shape of cells
5. Isolation of cell and tissue samples for culturing; cell separation methods
6. Primary cultures; monolayer, aggregate, explant and slide cultures;
7. Cell proliferation and apoptosis in vitro; assays on viability
8. Cell lines; establishment of cell lines from tissue samples; one-cell cloning; geno- and phenotypical stability
9. Cell banks; methods for cell-type identification
10. Intercellular communication in vitro; contact interactions; growth factors
11. Identification and extraction of cellular products from cultures
12. Adaptation of pharmacological experiments to cultures
13. Electrophysiological assays on cultivated cells
14. Gene-technological procedures on cultivated cells
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R. Ian Freshney: Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, John Wiley & Sons, 2011, ISBN 9780470649350
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John M. Davis: Animal Cell Culture: Essential Methods, John Wiley & Sons, 2011, ISBN 9780470975633
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Hand-outs of the lectures