In 4 separate blocks.
Block I (Days 1-3): Gene silencing (RNA interference) in genetic analysis. In silico (bioinformatics) design of primers, total RNA isolation, mRNA isolation, RT-PCR (reverse transcriptase PCR), cloning of a cDNA fragment into a „feeding” vektor (digestion, fragment purification, ligation), bacterial transformation. Phenotypic (phenocopy) analysis. Analysis of genetic pathway.
Block II (Days 4-6): Molecular techniques in genetic analysis I: PCR, Southern Blotting (detection of insertional, deletional and point mutations), Northern analysis (hybridization of ceh-20/bec-1 mRNA probes to RNA extracts from different developmental stages – developmental Northern).
Block III (Days 7-8): Western blotting (Detection of BEC-1 protein in different cell farctions – nucleus, organellum, cytoplasm). Genotyping with microsatellite markers.
Block IV (Days 9-10): Determining individual DNA patterns, sex determination.
Anthony J.F. Griffiths, Susan R. Wessler, Sean B. Carroll, John Doebley "An introduction to genetic analysis”, 10th edition, W. H. Freeman, 2010
SF Gilbert "Developmental Biology”, 7th edition, Palgrave Macmillan, 2003
EH Davidson "Genomic regulatory systems”, Academic Press 2002
B Lewin "Genes”, Jones & Bartlett Learning 2005